Response of intergeneric hybrids of Egyptian wheat with rye to in vitro techniques


Department of Genetics, Faculty of Agriculture, Minia University, El-Minia, Eg-61517, Egypt, Wide hybridization is necessary to generate variability that can be used in crop improvement. However, such crosses require that optimal conditions be availed to ensure successful embryo rescue. Progress on embryo rescue, callus induction and plant regeneration from intergeneric hybrids of two Egyptian wheat cultivars; the hexaploid aestivum wheat (Giza 163) and the tetraploid durum wheat (Sohag 1); with two diploid rye (Secale cereale L.) lines (Prolific and Imperial) are reported. In all crosses, rye was used as the pollen parent and hybrid pro-embryos were aseptically dissected 15-19 days after pollination and cultured on modified MS medium, supplemented with different concentrations and combinations of 2,4-D, IAA and kinetin. Results indicated that the genetic make up, age of the rescued embryo and the type of applied medium significantly influenced the induction of embryo and callus cultures. The examined Egyptian wheat cultivars are suitable for primary triticale production through embryo culture of their intergeneric hybrids with rye. The hybrid pro-embryos of hexaploid wheat x rye were much more responsive than those of tetraploid x rye. Embryo germination was observed only in Giza 163 x Prolific hybrid. Callusing was much more higher in media containing 2,4-D alone or with kinetin. Plant regeneration could beast be attained from callus cultures with MS medium supplemented with NAA and BAP (0.5 mg/l each). Some of the in vitro derived hybrids that grew up normally to maturity were fertile.

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